neb bsai hfv2 kit (New England Biolabs)
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New England Biolabs
neb bsai hfv2 kit
Neb Bsai Hfv2 Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+assembly/NEBridge+Golden+Gate+Assembly+Kit+(BsaI/pm42135436-238-23-27
Average 99 stars, based on 250 article reviews
Neb Bsai Hfv2 Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+assembly/NEBridge+Golden+Gate+Assembly+Kit+(BsaI/pm42135436-238-23-27
Average 99 stars, based on 250 article reviews
neb bsai hfv2 kit - by Bioz Stars,
2026-09
99/100 stars
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Cloning:Article Title: Compositions and methods for enhanced protein production in filamentous fungal cells Article Snippet: .. The seamless cloning is typically performed with, but not limited to, commercially available systems, such as Gibson Assembly (NEB), NEBuilder HiFi DNA Assembly (NEB), CRISPR:Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPR-Cas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210) using Construct:Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPR-Cas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210) using Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes. Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPRCas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210)45 using Synthesized:Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPR-Cas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210) using Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes. Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPRCas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210)45 using Article Title: Bridge recombinase enables versatile rewriting of bacterial genomes Article Snippet: .. Components were then assembled into complete pEdit vectors via Clone Assay:Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPR-Cas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210) using Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome Article Snippet: The Samy genome was isolated from the supernatant of S. ambofaciens ATCC 23877 grown in BM (bacteriophage medium), as previously described (Jaffal et al. ). .. This insert was then cloned into pOJ260-MoClo via Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome. Article Snippet: The Samy genome was isolated from the supernatant of S. ambofaciens ATCC 23877 grown in BM (bacteriophage medium), as previously described (Jaffal et al. 2024). .. This insert was then cloned into pOJ260-MoClo via Article Title: PEX11 mediates intralumenal vesicle formation in peroxisomes. Article Snippet: To target multiple PEX11 genes, GenScript synthesized constructs containing tandem PEX11A/B and PEX11C/D/E gRNAs flanked by BsaI cut sites. .. To generate CRISPRCas9 constructs, annealed sgRNA oligos or synthesized plasmids were cloned into BsaI sites of the pAGM55261 vector (Addgene 153210)45 using other:Article Title: Single-cell CRISPR activation screens in primary B cells discover gene regulatory mechanisms for hundreds of autoimmune risk loci Article Snippet: As controls, we designed sgRNAs for 10 loci with no TSS within 500 kb and no open chromatin signal in B cells within 30 kb (gene desert controls), 20 loci within 5-7 kb of any TSS and no open chromatin signal in B cells within 3 kb (distance-based controls), and three additional loci including CD5 and CD19 promoters and a non-coding regulatory element encoding rs74405933 upstream of CD83, in addition to 18 non-targeting sgRNAs from Calabrese Human CRISPR Activation Pooled Library ( ). |